Page 59 - Plastic News Issue August 2025
P. 59

BUSINESS NEWS








          Study Offers Enzymatic Recycling


          Cost Breakthrough




                                                                It is part of the PETzyme project, led by Profes-
                                                                sor José Manuel Martínez Costas (CiQUS) and Dr
                                                                Gemma Eibes (CRETUS).

                                                                Self-assembling spheres

                                                                Instead  of  purifying  enzymes  and  attaching
                                                                them to solid supports, the Spanish team used
                                                                a single-step process to embed the enzyme di-
                                                                rectly into nanoscale compartments that form
                                                                naturally within E. coli bacteria. These protein
                                                                structures are based on a viral protein known as
                cientists have developed a new technique        muNS-Mi, which self-assembles into spheres in-
                that could make enzymatic plastics recy-        side the bacterial cells.
          Scling more efficient and cost-effective by
          embedding enzymes inside bacterial protein            By tagging the enzyme with a short sequence, it
          compartments.                                         is automatically drawn into the protein compart-
                                                                ments during production. This IC-Tagging meth-
          The novel method, published in the Journal of         od eliminates the need for chromatography or
          Hazardous Materials, addresses a major hurdle         artificial carriers and results in enzymes that are
          in enzymatic recycling – the difficulty and cost      already immobilised and ready to use.
          of producing, isolating and reusing the enzymes
          that break down plastics such as PET.                 The  study focused  on a  genetically  optimised
                                                                version of the LCC (leaf-branch compost cuti-
          With PET widely used in bottles and food trays,       nase) enzyme, recognised for its ability to break
          enzymatic recycling is claimed to offer a cleaner     down PET efficiently. When tested on real post-
          alternative to traditional methods, which often       consumer plastics waste, including food packag-
          involve high temperatures or harsh chemicals.
                                                                ing and lab trays, the enzyme achieved over 90
          The work was carried out by researchers from          per cent depolymerisation in less than 72 hours.
          the Centre for Research in Biological Chemistry       It could also be reused in multiple cycles with lit-
          and Molecular Materials (CiQUS) and the Inter-        tle loss of activity.
          disciplinary Centre for Environmental Technolo-
          gies (CRETUS), both part of the University of         The  scientists  achieved  over  90  per  cent  en-
          Santiago de Compostela.                               zyme depolymerisation in less than 72 hours





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